C57BL/6-Cd3etm2(CD3E)Bcgen Il2ratm1(IL2RA)Bcgen/Bcgen • 121666
| Product name | B-hCD3E/hIL2RA mice |
|---|---|
| Catalog number | 121666 |
| Strain name | C57BL/6-Cd3etm2(CD3E)Bcgen Il2ratm1(IL2RA)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 12501,16184 |
| Aliases | CD3E, IMD18, T3E, TCRE; IL2RA,CD3e molecule; IL2RA, CD25, IDDM10, IL2R, IMD41, TCGFR, p55, interleukin 2 receptor subunit alpha |
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Targeting strategyGene targeting strategy for B-hCD3E/hIL2RA mice.
The exons 2-6 of mouse Cd3e gene that encode the extracellular domain were replaced by human CD3E exons 2-7 in B-hCD3E/hIL2RA mice. The exons 2-6 of mouse Il2ra gene that encode the extracellular domain were replaced by human IL2RA exons 2-6 in B-hCD3E/hIL2RA mice.
Protein expression analysis in T cells
Strain specific CD3E expression analysis in homozygous B-hCD3E/hIL2RA mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hIL2RA mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3E/hIL2RA mice (H/H;H/H) but not in WT mice (+/+).
Strain specific IL2RA expression analysis in homozygous B-hCD3E/hIL2RA mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hIL2RA mice (H/H;H/H) stimulated with anti-CD3ε in vivo (7.5 μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific anti-IL2RA antibody. Mouse IL2RA was detectable in WT mice (+/+). Human IL2RA was exclusively detectable in homozygous B-hCD3E/hIL2RA mice (H/H;H/H) but not in WT mice (+/+).
