C57BL/6N-Cd63tm1(CD63)Bcgen/Bcgen • 111103
| Product name | B-hCD63 mice |
|---|---|
| Catalog number | 111103 |
| Strain name | C57BL/6N-Cd63tm1(CD63)Bcgen/Bcgen |
| Strain background | C57BL/6N |
| NCBI gene ID | 12512 |
| Aliases | CD63(CD63 molecule), LAMP-3, ME491, MLA1, OMA81H, TSPAN30 |
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Targeting strategyGene targeting strategy for B-hCD63 mice.
The exons 2-8 of mouse Cd63 gene that encode the full-length protein were replaced by human CD63 exons 2-8 in B-hCD63 mice.
mRNA expression analysis
Strain specific analysis of CD63 gene expression in wild type (WT) and B-hCD63 mice by RT-PCR. Mouse Cd63 mRNA was detectable only in kidney of wild type mice. Human CD63 mRNA was detectable only in B-hCD63 mice but not in wild type mice.
Protein expression analysis
Strain specific CD63 expression analysis in homozygous B-hCD63 mice by flow cytometry.
Macrophages were collected from bone marrow of WT and homozygous B-hCD63 mice, and analyzed by flow cytometry with species-specific CD63 antibody. Mouse CD63 was detectable in WT mice. Human CD63 was exclusively detectable in homozygous B-hCD63 mice but not WT mice.
Strain specific CD63 expression analysis in homozygous B-hCD63 mice by flow cytometry.
Monocytes were collected from bone marrow of WT and homozygous B-hCD63 mice, and analyzed by flow cytometry with species-specific CD63 antibody. Mouse CD63 was detectable in WT mice. Human CD63 was exclusively detectable in homozygous B-hCD63 mice but not WT mice.
