Gene targeting strategy for B-hCD94 mice. The exon 3~6 of mouse Klrd1 gene that encode the extracellular domain were replaced by human KLRD1 exon 3~6 in B-hCD94 mice.

Strain specific CD94 expression analysis in homozygous B-hCD94 mice by flow cytometry. Splenocytes were collected from WT and heterozygous B-hCD94 (H/+) mice, and analyzed by flow cytometry with species-specific anti-CD94 antibody. Mouse CD94 was detectable in WT and heterozygous B-hCD94 (H/+) mice. Human CD94 was exclusively detectable in heterozygous B-hCD94 but not WT mice.