mRNA expression analysis
IHC analysis of EGFR expression
Blood routine test in B-hEGFR mice
Blood chemistry of B-hEGFR mice

Strain specific analysis of EGFR gene expression in wild type (WT) mice and B-hEGFR mice by RT-PCR.Mouse Egfr mRNA was detectable only in liver of WT mice (+/+). Human EGFR mRNA was detectable only in homozygous B-hEGFR mice (H/H) but not in WT mice (+/+).

Immunohistochemical (IHC) analysis of EGFR expression in homozygous B-hEGFR mice.The liver and kidney were collected from WT and homozygous B-hEGFR mice (H/H) and analyzed by IHC with anti-EGFR antibody. Mouse EGFR was detectable in WT mice, and human EGFR was detectable in homozygous B-hEGFR mice. The arrow indicates tissue cells with positive EGFR staining (brown).

Complete blood count (CBC). Blood from C57BL/6 and B-hEGFR mice (female, 9 week-old，n=8) was collected and analyzed for CBC. There was no differences among any measurement between C57BL/6 and B-hEGFR mice, indicating that introduction of hEGFR in place of its mouse counterpart does not change blood cell composition and morphology. Values are expressed as mean ± SEM.

Blood chemistry tests of B-hEGFR mice. Serum from the C57BL/6 and B-hEGFR mice (female, 9 week-old，n=8) was collected and analyzed for levels of ALT and AST. There was no differences on either measurement between C57BL/6 and B-hEGFR mice, indicating that introduction of hEGFR in place of its mouse counterpart does not change ALT and AST levels or health of liver. Values are expressed as mean ± SEM.