• CD42b is a 145 kD glycoprotein known as GpIbα. It is covalently bonded to CD42c to form GPIb.
• CD42b is expressed on platelets and megakaryocytes. CD42b/c heterodimer forms a complex with CD42a and d and acts as the receptor for von Willibrand factor and thrombin.

Gene targeting strategy for B-hGP1BA mice. The exon 2 of mouse Gp1ba gene that encode extracellular domain is replaced by human counterparts in B-hGP1BA mice. The promoter and 5’UTR and 3’UTR region, transmembrane and cytoplasmic domain of the mouse gene are retained. The chimeric GP1BA expression is driven by endogenous mouse Gp1ba promoter, while mouse Gp1ba gene transcription and translation will be disrupted.

Strain specific GP1RA expression analysis in homozygous B-hGP1BA mice by flow cytometry. Platelet and bone marrow was collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hGP1BA mice (H/H), and analyzed by flow cytometry with species-specific anti-human GP1BA/CD42b antibody (BD, 551061). Human GP1BA/CD42b was exclusively detectable in homozygous B-hGP1BA mice but not in wild-type mice.