C57BL/6-Erbb3tm1(ERBB3)Bcgen/Bcgen • 110791
| Product name | B-hHER3 mice |
|---|---|
| Catalog number | 110791 |
| Strain name | C57BL/6-Erbb3tm1(ERBB3)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| Aliases | ERBB3, ErbB-3, FERLK, HER3, LCCS2, MDA-BF-1, VSCN1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3 , erb-b2 receptor tyrosine kinase 3 |
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Targeting strategyGene targeting strategy for B-hHER3 mice. The exons 1-17 of mouse Her3 gene that encode the extracellular domain and transmembrane domain were replaced by human HER3 exons 1-17 in B-hHER3 mice.
mRNA expression analysis
Strain specific analysis of HER3 gene expression in wild type (WT) mice and B-hHER3 mice by RT-PCR. Mouse Her3 mRNA was detectable only in liver of WT mice (+/+). Human HER3 mRNA was detectable only in homozygous B-hHER3 mice (H/H) but not in WT mice (+/+).
IHC analysis of HER3 expression
Immunohistochemical (IHC) analysis of HER3 expression in homozygous B-hHER3 mice. The liver and colon were collected from WT and homozygous B-hHER3 mice (H/H) and analyzed by IHC with anti-HER3 antibody. HER3 was detectable in WT mice and homozygous B-hHER3 mice due to the cross-reactivity of the antibody. The arrow indicates tissue cells with positive HER3 staining (brown).
