C57BL/6N-Slc3a2tm1(SLC3A2)Rag2tm1/Bcgen • 112326
| Product name | B-hCD98HC/Rag2 KO mice |
|---|---|
| Catalog number | 112326 |
| Strain name | C57BL/6N-Slc3a2tm1(SLC3A2)Rag2tm1/Bcgen |
| Strain background | C57BL/6N |
| Aliases | SLC3A2, 4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE, CD98 heavy chain; RAG2, RAG-2, recombination activating 2 |
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Tageting strategyGene targeting strategy for B-hCD98HC/Rag2 KO mice.
The exons 2-10 of mouse Cd98 gene that encode the extracellular domain were replaced by human CD98 exons 4-12 in B-hCD98HC/Rag2 KO mice. The exon 3 encoding the full coding sequence and 3’UTR region of mouse Rag2 gene were knocked out in B-hCD98HC/Rag2 KO mice, resulting in a disruption of the Rag2 gene.
Protein expression analysis in brain endothelial cells
Strain specific CD98 expression analysis in homozygous B-hCD98/Rag2 KO mice by flow cytometry. Brain were collected from wild type (WT) mice (+/+) and homozygous B-hCD98HC/Rag2 KO mice (H/H;-/-), and analyzed by flow cytometry with species-specific anti-CD98 antibody after trypsin digestion. Mouse CD98 was detectable in WT mice (+/+). Human CD98 was exclusively detectable in homozygous B-hCD98HC/Rag2 KO mice (H/H;-/-) but not in WT mice (+/+).
Protein expression analysis in T cells
Strain specific CD98 expression analysis in homozygous B-hCD98HC/Rag2 KO mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD98HC/Rag2 KO mice (H/H;-/-), and analyzed by flow cytometry with species-specific anti-CD98 antibody. Mouse CD98 was detectable in WT mice (+/+) and human CD98 was not detectable in WT mice (+/+). There were no T cells in homozygous B-hCD98HC/Rag2 KO mice.
Protein expression analysis in B cells
Strain specific CD98 expression analysis in homozygous B-hCD98HC/Rag2 KO mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD98HC/Rag2 KO mice (H/H;-/-), and analyzed by flow cytometry with species-specific anti-CD98 antibody. Mouse CD98 was detectable in WT mice (+/+) and human CD98 was not detectable in WT mice (+/+). There were no B cells in homozygous B-hCD98HC/Rag2 KO mice.
Protein expression analysis in NK cells
Strain specific CD98 expression analysis in homozygous B-hCD98HC/Rag2 KO mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD98HC/Rag2 KO mice (H/H;-/-), and analyzed by flow cytometry with species-specific anti-CD98 antibody. Mouse CD98 was detectable in WT mice (+/+). Human CD98 was exclusively detectable in homozygous B-hCD98HC/Rag2 KO mice (H/H;-/-) but not in WT mice (+/+).
