B-Bcrp KO mice

C57BL/6N-Abcg2tm1Bcgen/Bcgen • 112776

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B-Bcrp KO mice

Product nameB-Bcrp KO mice
Catalog number112776
Strain nameC57BL/6N-Abcg2tm1Bcgen/Bcgen
Strain backgroundC57BL/6N
AliasesABC15, ABCP, BCRP, Bcrp1, MXR, MXR1
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  • Targeting strategy
  • Phenotypic analysis
Targeting strategy

Gene targeting strategy for B-Bcrp KO mice. The exons 2~16 of mouse Abcg2 gene were knocked out in B-Bcrp KO mice, resulting in a disruption of the Abcg2 gene.

mRNA expression analysis
Strain specific analysis of Abcg2 mRNA expression in wild-type C57BL/6 mice and B-Bcrp KO mice by RT-PCR. Brain and kidney RNA were isolated from wild-type C57BL/6 mice (+/+) and B-Bcrp KO mice (-/-), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Abcg2 primers. Mouse Abcg2 mRNA was detectable in wild-type C57BL/6 mice (+/+) but not in B-Bcrp KO mice (-/-).
Protein expression analysis

Western blot analysis of BCRP protein expression in B-Bcrp KO mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and B-Bcrp KO mice (-/-), and then analyzed by western blot with anti-BCRP antibody. 40 μg total proteins were loaded for western blotting analysis. BCRP was detectable in kidney, liver, heart of wild-type C57BL/6 mice (+/+) but not B-Bcrp KO mice (-/-).