Basic Information

Common Name
B-hCD20 MC38
Aliases
MS4A1, Bp35, CVID5
Catalog Number
310716
Cell Type/ Disease
Colon Carcinoma
Species/ Strain
Mus musculus, C57BL/6
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Description

The human CD20 coding sequence was inserted into the ROSA26 locus in MC38 cells. Human CD20 is highly expressed on the surface of B-hCD20 MC38 cells.

Targeting strategy

The exogenous promoter and human CD20 coding sequence was inserted into the ROSA26 locus in murine.

Protein expression analysis

CD20 expression analysis in B-hCD20 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCD20 MC38 cultures were stained with species-specific anti-CD20 antibody. Human CD20 was detected on the surface of B-hCD20 MC38 cells but not wild-type MC38 cells. The 2-A12 clone of B-hCD20 MC38 cells was used for in vivo experiments.

Tumor growth curve

Subcutaneous homograft tumor growth of B-hCD20 MC38 cells. B-hCD20 MC38 cells (1×107) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6N mice (female, 8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD20 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

The subcutaneous tumor formation of B-hCD20 MC38 cells was slow, and the inoculation dose was recommended to be increased to 1×107.