Basic Information

Common name
B-hCD20 EL4
Catalog number
310717
Aliases
MS4A1, Bp35, CVID5
Disease
Lymphoma
Organism
Mouse
Strain
C57BL/6N
Tissue types
Lymphoma
Tissue
T lymphocyte
Application
B-hCD20 EL4 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Description

The human CD20 coding sequence was inserted into the ROSA26 locus in EL4 cells. Human CD20 is highly expressed on the surface of B-hCD20 EL4 cells.

Targeting strategy

Gene targeting strategy for B-hCD20 EL4 cells. The exogenous promoter and human CD20 coding sequence was inserted into the ROSA26 locus in murine.

Protein expression analysis

CD20 expression analysis in B-hCD20 EL4 cells by flow cytometry. Single cell suspensions from wild-type EL4 and B-hCD20 EL4 cultures were stained with species-specific anti-CD20 antibody. Human CD20 was detected on the surface of B-hCD20 EL4 cells but not wild-type EL4 cells. The 2-E06 clone of B-hCD20 EL4 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hCD20 EL4 cells. B-hCD20 EL4 cells (2×105) and wild-type EL4 cells (2×105) were subcutaneously implanted into C57BL/6N mice (female, 6-9-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD20 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies.

Animals with tumor volume over 3000mm3 in the control group were euthanized.

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