Homozygousmiceforthetargetedmutationareviable,fertile,normalinsizeanddonotdisplayanygrossphysicalorbehavioralabnormalities

NPY deletion in osteocytes rather than in osteoblasts of Dmp1-iCre (Dmp1-iCre was purchased from Biocytogen Co. Ltd., Beijing, China); Npyfl/fl mice, was confirmed by western blotting for the protein lysates of the isolated osteocytes and osteoblasts (A).

NPY protein expression was much lower in the bone marrow-depleted femur homogenates, but not in the whole bone marrow cells, the whole brain lysate, and the NPY-abundant hypothalamus, of 4-month-old Dmp1-iCre; Npyfl/fl mice than that of Npyfl/fl littermates (C).

Gel image of PCR products. PCR products are separated by gel electrophoresis on a 2% agarose gel. PC: samples used for PCR were collected from heterozygous mice; WT: samples used for PCR were collected from wild type mice; H₂O: blank control.