B-Drd2-Cre rats

SD-Drd2tm1(cre)Bcgen/Bcgen • 210488

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B-Drd2-Cre rats

Product nameB-Drd2-Cre rats
Catalog number210488
Strain nameSD-Drd2tm1(cre)Bcgen/Bcgen
Strain backgroundSD
NCBI gene ID24318
Official symbolDrd2 (dopamine receptor D2), D2R; Drd-2
Chromosome8
ApplicationFunction researchof genes This Drd2Cre model is an efficienttool to study various gene functions when crossed with rats with different loxPsite-flanked genes of interest, especially in studies of D2 dopamine receptorsubtype-expressing neurons, and may be useful for studying dopamine-sensitivebehaviors such as addiction.
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  • Description
  • Targeting strategy
  • Phenotypic analysis
Description
  • Function research of genes
  • This Drd2Cre model is an efficient tool to study various gene functions when crossed with rats with different loxP site-flanked genes of interest, especially in studies of D2 dopamine receptor subtype-expressing neurons, and may be useful for studying dopamine-sensitive behaviors such as addiction. 
Gene editing strategy
A P2A-NLS-Cre sequence cassette was placed between the coding sequence of exon 3 and 3’UTR of the Drd2 gene. This strain was maintained on a Sprague Dawley genetic background.
Phenotype Analysis

k Immunofluorescent images of tdTomato and DRD2 in the striatum of Drd2 reporter rats. Arrows indicate the colocalization of tdTomato and DRD2. Scale bar, 25 µm. l Western blot of DRD2 and GAPDH in the striatum from Drd2::Cre+/− and WT rats. Left, representative blots, right, quantifcation results. NS not signifcant, n= 3, t test (source: Yu Q et al.,2019)