Gel image of PCR products. PCR products are separated by gel electrophoresis on a 2% agarose gel. PC:samples used for PCR were collected fromheterozygousmice; WT:samples used for PCRwere collected fromwild typemice; H₂O: blank control.

Scheme for generating Wnt7bRANK mice. Rosa26-Wnt7b mice were generated and crossed with RANK-Cre mice (RANK-Cre was purchased from Biocytogen Co. Ltd., Beijing, China) to obtain Wnt7bRANK mice. Wnt7bRANK mice similar to the overexpression of Wnt7b in osteoblasts. the three-dimensional reconstruction of mCT scanning showed a significant increase in bone mass in both trabecular and cortical bone in Wnt7bRANK mice compared to their littermates (Rosa26Wnt7b,hereafter referred to as wildtype or WT).(source: Wu F et al., 2021)