C57BL/6JNifdc-Ldlrtm1Bcgen/Bcgen • 113270
| Product name | B-Ldlr KO mice |
|---|---|
| Catalog number | 113270 |
| Strain name | C57BL/6JNifdc-Ldlrtm1Bcgen/Bcgen |
| Strain background | C57BL/6JNifdc |
| NCBI gene ID | 3949 |
| Aliases | FH, FHC, FHCL1, LDLCQ2 |
Description
Targeting strategyGene targeting strategy for B-Ldlr KO mice. The exons 4-18 of mouse Ldlr gene were knocked out in B-Ldlr KO mice, resulting in a disruption of the Ldlr gene.
mRNA expression analysis in humanized B-Ldlr KO mice
Species specific analysis of Ldlr gene expression in wild-type C57BL/6JNifdc mice and homozygous B-Ldlr KO mice by RT-PCR. Liver was collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-Ldlr KO mice (-/-). Mouse Ldlr mRNA was detectable only in wild-type C57BL/6JNifdc mice, but not in homozygous B-Ldlr KO mice.
Protein expression analysis
Western blot analysis of LDLR protein expression in homozygous B-Ldlr KO mice. Various tissue lysates were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-Ldlr KO mice (-/-), and then analyzed by western blot with anti-mouse LDLR antibody (R&D, AF2255). 40 μg total proteins were loaded for western blotting analysis. LDLR was only detected in wild-type mice, but not in homozygous B-Ldlr KO mice.
Analysis of lipid metabolism after Ldlr knockout in B-Ldlr KO mice 
Lipid metabolism analysis in B-Ldlr KO mice. Serum concentrations of TG, TC, LDL-C, and HDL-C were analyzed in 4-hour-fasted B-Ldlr KO mice (-/-) and wild-type C57BL/6JNifdc mice (+/+) (6 weeks old). The levels of LDL-C, TC, and TG in B-Ldlr KO mice were significantly higher than those in wild-type C57BL/6JNifdc mice.
TG, triglycerides; TC, total cholesterol; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol; F, female; M, male.
Values are expressed as mean ± SEM. Significance was determined by a two-way ANOVA test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
