C57BL/6JNifdc-Ldlrtm1Bcgen/Bcgen • 113270
Product name | B-Ldlr KO mice |
---|---|
Catalog number | 113270 |
Strain name | C57BL/6JNifdc-Ldlrtm1Bcgen/Bcgen |
Strain background | C57BL/6JNifdc |
NCBI gene ID | 3949 |
Aliases | FH, FHC, FHCL1, LDLCQ2 |
Gene targeting strategy for B-Ldlr KO mice. The exons 4-18 of mouse Ldlr gene were knocked out in B-Ldlr KO mice, resulting in a disruption of the Ldlr gene.
Species specific analysis of Ldlr gene expression in wild-type C57BL/6JNifdc mice and homozygous B-Ldlr KO mice by RT-PCR. Liver was collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-Ldlr KO mice (-/-). Mouse Ldlr mRNA was detectable only in wild-type C57BL/6JNifdc mice, but not in homozygous B-Ldlr KO mice.
Western blot analysis of LDLR protein expression in homozygous B-Ldlr KO mice. Various tissue lysates were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-Ldlr KO mice (-/-), and then analyzed by western blot with anti-mouse LDLR antibody (R&D, AF2255). 40 μg total proteins were loaded for western blotting analysis. LDLR was only detected in wild-type mice, but not in homozygous B-Ldlr KO mice.