B-hANXA2 MC38

• 321942

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B-hAMHR2 MC38
B-hAREG MC38

B-hANXA2 MC38

Product nameB-hANXA2 MC38
Catalog number321942
Strain backgroundC57BL/6
AliasesP36; ANX2; LIP2; LPC2; CAL1H; LPC2D;ANX2L4; PAP-IV; HEL-S-270
TissueColon
DiseaseColon carcinoma
SpeciesMouse
ApplicationB-hANXA2 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
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  • Description
  • Targeting strategy
  • Phenotypic analysis

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Description

The mouse Anxa2 gene was replaced by human ANXA2 coding sequence in B-hANXA2 MC38 cells. Human ANXA2 is highly expressed on the surface of B-hANXA2 MC38 cells.

Targeting strategy

Gene targeting strategy for B-hANXA2 MC38 cells. The exogenous promoter and human ANXA2 coding sequence was inserted to replace part of murine exon 3. The insertion disrupts the endogenous murine Anxa2 gene, resulting in a non-functional transcript.

Protein expression analysis

ANXA2 expression analysis in B-hANXA2 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hANXA2 MC38 cultures were stained with anti-ANXA2 antibody. Human ANXA2 was detected on the surface of B-hANXA2 MC38 cells and MC38 cells,  as the antibody is crossly reactive with ANXA2 in human and mice. It has been verified that B-hANXA2 MC38 cells do not express mouse mRNA by RT-PCR. The 2-A01 clone of B-hANXA2 MC38 cells was used for in vivo experiments.

Subcutaneous homograft tumor growth of B-hANXA2 MC38 cells. B-hANXA2 MC38 cells (1x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diaANXA2er X short diaANXA2er2. As shown in panel A, B-hANXA2 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.