B-hB7-H3 CT26.WT

• 321829

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B-hB7-H3 CT26.WT

Product nameB-hB7-H3 CT26.WT
Catalog number321829
Strain backgroundBALB/c
AliasesCD276; B7H3; B7RP-2; 4Ig-B7-H3
TissueColon
DiseaseColon carcinoma
SpeciesMouse
ApplicationB-hB7-H3 CT26.WT cells have the capability to establish tumors in vivo
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  • Description
  • Targeting strategy
  • Phenotypic analysis
  • Tumorigenicity

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Description

The mouse B7-h3 gene was replaced by human B7-H3 coding sequence in B-hB7-H3 CT26.WT cells. Human B7-H3 is highly expressed on the surface of B-hB7-H3 CT26.WT cells.

Targeting strategy

Gene targeting strategy for B-hB7-H3 CT26.WT cells. The chemic human and mouse B7-H3 coding sequence were inserted to replace part of murine exon 3 and exon 4. The insertion disrupts the endogenous murine B7-h3 gene, resulting in a non-functional transcript.

Protein expression analysis

B7-H3 expression analysis in B-hB7-H3 CT26.WT cells by flow cytometry. Single cell suspensions from B-hB7-H3 CT26.WT cultures were stained with species-specific anti-B7-H3 antibody. Human B7-H3 was detected on the surface of B-hB7-H3 CT26.WT cells, but not on the surface of wild-type CT26.WT cells. The 3-H02 clone of B-hB7-H3 CT26.WT cells was used for in vivo experiments.

Protein expression analysis of tumor cells
B-hB7-H3 CT26.WT cells were subcutaneously transplanted into BALB/c mice (n=5). At the end of the experiment, tumor cells were harvested and assessed for human B7-H3 expression by flow cytometry. As shown, human B7-H3 was highly expressed on the surface of tumor cells. B-hB7-H3 CT26.WT cells can be used for in vivo efficacy studies of novel B7-H3 therapeutics.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hB7-H3 CT26.WT cells. B-hB7-H3 CT26.WT cells and wild-type CT26.WT cells were subcutaneously implanted into BALB/c mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hB7-H3 CT26.WT cells were able to establish tumors in vivo and can be used for efficacy studies.