B-hUPAR MC38

• 311651

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B-hUPAR MC38

Product nameB-hUPAR MC38
Catalog number311651
Strain backgroundC57BL/6
AliasesCD87; UPAR; URKR; U-PAR;PLAUR
TissueColon
DiseaseColon carcinoma
SpeciesMouse
ApplicationB-hUPAR MC38 cells have the capability to establish tumors in vivo
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  • Description
  • Targeting strategy
  • Phenotypic analysis
  • Tumorigenicity

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Description

The mouse Upar gene was replaced by human UPAR coding sequence in B-hUPAR MC38 cells. Human UPAR is highly expressed on the surface of B-hUPAR MC38 cells.

Targeting strategy

Gene targeting strategy for B-hUPAR MC38 cells. The exogenous promoter and chimeric CDS containing mouse signal peptide and human UPAR coding sequence were inserted to replace part of mouse Upar. The insertion disrupts the endogenous murine Upar gene, resulting in a non-functional transcript.

Protein expression analysis

UPAR expression analysis in B-hUPAR MC38 cells by flow cytometry. Single cell suspensions from B-hUPAR MC38 cultures were stained with species-specific anti-UPAR antibody. Human UPAR was detected on the surface of B-hUPAR MC38 cells, but not on the surface of wild-type MC38 cells. The 5-F05 clone of B-hUPAR MC38 cells was used for in vivo experiments.

Protein expression analysis of tumor cells

Tumor cells were harvested and assessed for human UPAR expression by flow cytometry. As shown, human UPAR was highly expressed on the surface of tumor cells. Therefore, B-hUPAR MC38 cells can be used for in vivo efficacy studies of novel UPAR therapeutics.

Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hUPAR MC38 cells. B-hUPAR MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into B-hUPAR mice (male, 7-9-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hUPAR MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
B-hUPAR MC38 cells were subcutaneously transplanted into B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice and C57BL/6 (n=6), and on 25 days post inoculation, tumor cells were harvested and assessed for human UPAR expression by flow cytometry. As shown, human UPAR was highly expressed on the surface of tumor cells. Therefore, B-hUPAR MC38 cells can be used for in vivo efficacy studies of novel UPAR therapeutics.