C57BL/6-Ctla4tm1(CTLA4)Bcgen Tnfr2tm1(TNFR2)Bcgen/Bcgen • 111843
| Product name | B-hCTLA4/hTNFR2 mice |
|---|---|
| Catalog number | 111843 |
| Strain name | C57BL/6-Ctla4tm1(CTLA4)Bcgen Tnfr2tm1(TNFR2)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| Aliases | CTLA4:CTLA-4; ALPS5; GRD4; CTLA4; CD; CD152; IDDM12; GSE; CELIAC3TNFR2: p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II |
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Targeting strategyGene targeting strategy for B-hCTLA4/hTNFR2 mice. The exon 2 of mouse Ctla4 gene that encodes the extracellular domain was replaced by human CTLA4 exon 2 in B-hCTLA4/hTNFR2 mice. The exons 2~6 of mouse Tnfr2 gene that encode the extracellular domain was replaced by human TNFR2 exons 2~6 in B-hCTLA4/hTNFR2 mice.
Protein expression analysis
Strain specific hCTLA4 expression analysis in homozygous B-hCTLA4/hTNFR2 mice by flow cytometry. Spleen was collected from wild-type mice (+/+) and homozygous B-hCTLA4/hTNFR2 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD152 (CTLA4) antibodies. Mouse CTLA4 was detectable in wild-type mice. Human CTLA4 was exclusively detectable in homozygous B-hCTLA4/hTNFR2 mice but not in wild-type mice.
Strain specific hTNFR2 expression analysis in homozygous B-hCTLA4/hTNFR2 mice by flow cytometry. Spleen was collected from wild-type mice (+/+) and homozygous B-hCTLA4/hTNFR2 mice (H/H), and analyzed by flow cytometry with species-specific anti-TNFR2 antibodies. Mouse TNFR2 was detectable in wild-type mice. Human TNFR2 was exclusively detectable in homozygous B-hCTLA4/hTNFR2 mice but not in wild-type mice.
