Although Biocytogen has the most advanced gene targeting technology and capacity, we still can meet some customers’ special needs for random insertion transgene requests.
The Difference Between Genome Editing Technology and Transgenic Technology
Both Genome editing technology and transgenic techniques can alter the genome of an organism so that the desirable trait can be inherited, but there is a big difference between the two. Genome editing is the targeted manipulation of the genome of the organism itself by knocking out or replacing targeted gene which results in individuals with intentionally selected and desired traits, while transgenic technology introduces biologically nonexisting foreign genes to the original organisms in order to tailor the species with new traits.
Generation of animal lines through random integration, transgenesis, can lead to several confounding effects on gene expression, including multiple integrations per cell, the activation or disruption of endogenous genes at or near the site of integration, and unstable expression of the transgene due to epigenetic modifications. These, coupled with the constitutive and often high expression levels of the transgene can lead to unpredictable cell behavior and irreproducible or hard to interpret results.
Targeted gene editing overcomes these limitations by introducing modifications at the endogenous locus of the gene (and only this site), such that they are expressed from the native promoter. Consequently, the effect of gain of function can be studied in a physiological context.
Tol2 Transposon System:
The Tol2 transposon system, originally used in zebrafish, is used to make transgenic mice. Transgene-mediated insertions of the Tol2 system are prone to be at the A/T rich region so that the expression of the transgene is more efficient. Compared with traditional DNA pronuclear injection, the Tol2 system uses cytoplasmic injection, which is easy to operate and has a high success rate. Using our Tol2 system, we can deliver transgenes up to 30kb in mice.
BAC transgenic technology is the first technology to add exogenous genes to the host species and was widely applied to many experimental organisms and economical organisms such as crops, livestock.