Active across all known vertebrates and originally discovered in zebrafish, the Tol2 transposon system can be used to make transgenic mouse models. Via transposase catalyzation, a transposon construct can be effectively inserted before or after the Tol2 gene with a cut-and-paste mechanism. With little chance of rearrangement or modification at the target site, the Tol2 transgenic model has many potential applications. Mainly, the transgene-mediated insertions of the Tol2 system are prone to be at the A/T rich region, which makes the expression of the transgene more efficient, and can increase the rate of gene integration as well as integrate foreign genes into A/T rich regions. Compared with traditional DNA pronuclear injection, the Tol2 system uses cytoplasmic injection, which is easy to operate and has a high success rate. Using our Tol2 system, we can deliver transgenes up to 30kb in mice.
BAC transgenic technology is commonly used to add exogenous genes to the host species and has been widely applied to many experimental and economical organisms such as crops/livestock.
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