Gene editing (or Genome Editing) is a technique used to make specific changes to the DNA of an organism. This technique is commonly used to create gene deletions and insertions (e.g. point mutations). Unlike transgenesis, gene editing allows for permanent alterations in the mouse genome, and these can occur globally (whole-body) or in a tissue-specific manner.
In the past, scientists relied primarily on embryonic stem cell (ESC) based gene targeting to produce knockout (KO) and knockin (KI) mice. Homologous recombination (HR) between a targeting vector and ESC genomic DNA (ESC/HR) results in ESCs containing a targeted mutation. Targeted ESCs are microinjected into blastocysts, which are then implanted into foster animals to ultimately produce founder mice. However, this technology is limited to mice because only mouse germline transmittable ESCs are available. The recently developed CRISPR/Cas9 based gene editing is becoming the gold standard in gene editing based on its efficiency and versatility.
Biocytogen has developed innovative Extreme Genome Editing — or EGE™ — system, which is based on CRISPR/Cas9. EGE™ is up to 20-fold more efficient at knocking in large DNA fragments compared to CRISPR/Cas9 alone, and is ideal for generating mouse/rat genetic models and custom cell lines. As a result, EGE™ minimizes the timeline to generate gene edited animal models and is successful on 98% of Biocytogen’s projects.
Biocytogen’s team of experts employ strict quality controls when generating mouse, rat, and cell line models. Therefore, you can have confidence in the models we deliver to you and focus on downstream applications. A dedicated project manager will provide monthly updates on the status of your project.
Why Gene Editing Is Important
Gene editing is a powerful tool that allows researchers to study the function of genes and to associate gene mutations with physiological and pathological phenotypes. Gene editing is also used to generate animal models of disease which can lead to the development of novel therapies. CRISPR/Cas9 based gene editing is employed to create humanized animal models which are widely used for drug screening, organ transplantation, diagnostics, and personalized treatments, most notably in the field of oncology.
Biocytogen — World-Class Gene Editing Services
In the past decade, Biocytogen has grown into a global leader in the field of gene editing and animal model generation services. Using our cutting-edge technology platforms, we offer customized animal models and a vast collection of gene humanized mouse models for research and drug screening/evaluation. We generate approximately 1,500 mouse/rat models every year and ship to our customers worldwide. Biocytogen provides outstanding service, quick turnaround times and a 100% money back guarantee. For more information about our gene editing and animal generation services, contact us today.