Immunodeficient (B-NDG) Mice

Humanized immunodeficient mice offer a versatile model for the assessment of human-based immunotherapies. Our team at Biocytogen independently designed and generated the severely immunodeficient B-NDG mouse model (NOD-Prkdc^scidIL2rg^tm1/Bcgen). By deleting the IL2rg gene from the NOD-scid genetic background, the resulting B-NDG mice lack mature T cells, B cells, functional NK cells, and display cytokine signaling deficiencies. These collective properties render B-NDG mice with the highest degree of immunodeficiency, making B-NDG mice most suitable for engraftment and growth of human hematopoietic stem cells (HSCs), peripheral blood mononuclear cells (PBMCs), and human tumor cells/tissues. 

• Prkdc null mice (scid): deficient in functional T cells and B cells, as well as an absence of lymphocytes, altogether recapitulating the severe combined immunodeficiency (scid) phenotype seen in human patients. 
• NOD-scid mice: NOD mice combined with Prkdc gene knockout results in a loss of functional T cells, B cells and the complement system, in addition to reducing the activity of NK cells. 
• IL2rg null mice: disruption of the IL2rg gene leads to impaired immune function, characterized by reduced T and B cells, and loss of NK cells. 

Advantages of B-NDG Mice

• Model currently holds the highest degree of immunodeficiency among all immunodeficiency models
• Longer lifespan than NOD-scid or NSG mice; 1.5 years on average
• Minimal to absent rejection of human-derived cells and tissues
• More efficient for CDX model generation
• No B lymphocyte leakage

Major Applications

• Human-derived cell or tissue engraftment
• Tumor and tumor stem cell research
• ES and iPS cell research
• Hematopoiesis and immunology studies
• Human infectious disease studies
• Development of humanized models

B-NDG Mice

Product	Product Name	Catalog Number	Application area
B-NDG Mice	B-NDG	110586	Immune reconstitution, CDX, transplantation, drug evaluation

B-NDG-Based Mouse Models

Product	Product No	Background	Application area
B-Fcgr4 KO mice(CB-17 SCID)	111654	CB-17 SCID
B-NDG B2m KO mice plus	110601	B-NDG	Reduce PBMC reconstitution GVHD response and prolong experimental window
B-NDG B2m KO plus/hIL15 mice	111856	B-NDG	Human immune system reconstitution
B-NDG H2-Ab1 KO mice	110595	B-NDG	No MHC class II molecule expression, Reduce GVHD and prolong the experimental window period, Improve the accuracy of efficacy verification
B-NDG HLA-A2.1 mice	110599	B-NDG	Vaccine study
B-NDG Hc mice	110605	B-NDG	Antibody CDC function study
B-NDG MGMT3 mice	111882	B-NDG
B-NDG MHC I/II DKO mice plus	111895	B-NDG	No MHC class I/II molecule expression, Reduce GVHD and prolong the experimental window period, Improve the accuracy of efficacy verification
B-NDG hALB mice	111982	B-NDG
B-NDG hB2M/HLA-A2.1 mice plus	112449	B-NDG
B-NDG hCSF1/hTHPO mice	120609	B-NDG	Promote differentiation of human myeloid cells without irradiation
B-NDG hFcRn mice	111922	B-NDG	PK (NDG background can reduce ADA)
B-NDG hGM-CSF mice	110593	B-NDG	Promote differentiation of human myeloid cells, For mating with other humanized mice that promote myeloid cell differentiation to obtain multi-humanized mice
B-NDG hIL15 mice	110600	B-NDG	Promote human NK cell differentiation
B-NDG hIL15, FcγR KO mice	112653	B-NDG
B-NDG hIL6 mice	110596	B-NDG	Promotes human monocyte differentiation
B-NDG hIL7 mice	110597	B-NDG	l Promote differentiation of human lymphoid cells
B-NDG hIL7/hIL15 mice	120612	B-NDG	Promote differentiation of human T cells and NK cells.
B-NDG hSIRPA mice	110604	B-NDG	Maintain progenitor capacity
B-NDG hSIRPA/hCD47 mice	110603	B-NDG	Evaluation of pharmacodynamic and toxicity of CD47, SIRPA targeted drugs
B-NDG hTHPO mice	110590	B-NDG	Without irradiation, it reduces the damage caused by irradiation to mice
B-NDG hairless mice	110598	B-NDG	l Hairless, easy to measure tumor
B-NDG nude mice	110587	B-NDG	Human immune system reconstitution
B-Rag2 KO mice	110809	C57BL/6N	l No mature T or B cellsl Used for efficacy and toxicity verification
B-hCD98HC/Rag2 KO mice	112326	C57BL/6N	Used for verification of efficacy and toxicity of drugs targeting human CD98HC
hPBMC-B-NDG mice	112469	NOD scid	Human T cell reconstitution, Human-derived cell or tissue engraftment, Tumor research, Human disease model study, Efficacy and toxicity verification for human targeted drugs
huHSC-B-NDG hIL15 mice	112478	B-NDG
huHSC-B-NDG mice	112470	NOD scid	Human immune system reconstitution, mainly T cells and B cells, reconstitution Human-derived cell or tissue engraftment Tumor research, Human disease model study, Efficacy and toxicity verification
huNK-B-NDG hIL15 mice	112482	B-NDG