Humanized immunodeficient mice offer a versatile model for the assessment of human-based immunotherapies. Our team at Biocytogen independently designed and generated the severely immunodeficient B-NDG mouse model (NOD-PrkdcscidIL2rgtm1/Bcgen). By deleting the IL2rg gene from the NOD-scid genetic background, the resulting B-NDG mice lack mature T cells, B cells, functional NK cells, and display cytokine signaling deficiencies. These collective properties render B-NDG mice with the highest degree of immunodeficiency, making B-NDG mice most suitable for engraftment and growth of human hematopoietic stem cells (HSCs), peripheral blood mononuclear cells (PBMCs), and human tumor cells/tissues.
- Prkdc null mice (scid): deficient in functional T cells and B cells, as well as an absence of lymphocytes, altogether recapitulating the severe combined immunodeficiency (scid) phenotype seen in human patients.
- NOD-scid (or NSG) mice: NOD mice combined with Prkdc gene knockout results in a loss of functional T cells, B cells and the complement system, in addition to reducing the activity of NK cells.
- IL2rg null mice: disruption of the IL2rg gene leads to impaired immune function, characterized by reduced T and B cells, and loss of NK cells.
Advantages of B-NDG Mice
- Model currently holds the highest degree of immunodeficiency among all immunodeficiency models
- Longer lifespan than NOD-scid or NSG mice; 1.5 years on average
- Minimal to absent rejection of human-derived cells and tissues
- More efficient for CDX model generation
- No B lymphocyte leakage
- Human-derived cell or tissue engraftment
- Tumor and tumor stem cell research
- ES and iPS cell research
- Hematopoiesis and immunology studies
- Human infectious disease studies
- Development of humanized models
B-NDG-Based Mouse Models