Immunodeficient (B-NDG) Mice

Humanized immunodeficient mice offer a versatile model for the assessment of human-based immunotherapies. Our team at Biocytogen independently designed and generated the severely immunodeficient B-NDG mouse model(NOD-PrkdcscidIL2rgtm1/Bcgen). By deleting the IL2rg gene from the NOD-scid genetic background, the resulting B-NDG mice lack mature T cells, B cells, functional NK cells, and display cytokine signaling deficiencies. These collective properties render B-NDG mice with the highest degree of immunodeficiency, making B-NDG mice most suitable for engraftment and growth of human hematopoietic stem cells (HSCs), peripheral blood mononuclear cells (PBMCs), and human tumor cells/tissues. 

  • Prkdc null mice (scid): deficient in functional T cells and B cells, as well as an absence of lymphocytes, altogether recapitulating the severe combined immunodeficiency (scid) phenotype seen in human patients. 
  • NOD-scid mice: NOD mice combined with Prkdc gene knockout results in a loss of functional T cells, B cells and the complement system, in addition to reducing the activity of NK cells. 
  • IL2rg null mice: disruption of the IL2rg gene leads to impaired immune function, characterized by reduced T and B cells, and loss of NK cells. 

Advantages of B-NDG Mice

  • Model currently holds the highest degree of immunodeficiency among all immunodeficiency models
  • Longer lifespan than NOD-scid or NSG mice; 1.5 years on average
  • Minimal to absent rejection of human-derived cells and tissues
  • More efficient for CDX model generation
  • No B lymphocyte leakage

Major Applications

  • Human-derived cell or tissue engraftment
  • Tumor and tumor stem cell research
  • ES and iPS cell research
  • Hematopoiesis and immunology studies
  • Human infectious disease studies
  • Development of humanized models

B-NDG Mice

Product Product Name Catalog Number Application area
B-NDG Mice B-NDG 110586 Immune reconstitution, CDX, transplantation, drug evaluation

B-NDG-Based Mouse Models

Product Product No Background Application area
B-Fcgr4 KO mice(CB-17 SCID) 111654 CB-17 SCID
B-NDG B2m KO mice plus 110601 B-NDG Reduce PBMC reconstitution GVHD response and prolong experimental window
B-NDG B2m plus/hIL15 mice 111856 B-NDG Human immune system reconstitution
B-NDG H2-Ab1 KO mice 110595 B-NDG No MHC class II molecule expression, Reduce GVHD and prolong the experimental window period, Improve the accuracy of efficacy verification
B-NDG H2-K1/H2-D1 KO mice 112256 B-NDG
B-NDG HLA-A2.1 mice 110599 B-NDG Vaccine study
B-NDG Hc mice 110605 B-NDG Antibody CDC function study
B-NDG MGMT3 mice 111882 B-NDG
B-NDG MHC I/II DKO mice plus 111895 B-NDG No MHC class I/II molecule expression, Reduce GVHD and prolong the experimental window period, Improve the accuracy of efficacy verification
B-NDG hALB mice 111982 B-NDG
B-NDG hALB/hFcRn mice 112774 B-NDG
B-NDG hB2M/HLA-A11.1 mice 112451 B-NDG
B-NDG hB2M/HLA-A2.1 mice plus 112449 B-NDG
B-NDG hB2M/HLA-A24.2 mice 112560 B-NDG
B-NDG hB2M/HLA-A24.2 mice plus 112874 B-NDG
B-NDG hB2M/HLA-A3.1 mice 112450 B-NDG
B-NDG hB2M/HLA-A3.1 mice plus 112873 B-NDG
B-NDG hCSF1/hTHPO mice 120609 B-NDG Promote differentiation of human myeloid cells without irradiation
B-NDG hFcRn mice 111922 B-NDG PK (NDG background can reduce ADA)
B-NDG hGM-CSF mice 110593 B-NDG Promote differentiation of human myeloid cells, For mating with other humanized mice that promote myeloid cell differentiation to obtain multi-humanized mice
B-NDG hIL15 mice 110600 B-NDG Promote human NK cell differentiation
B-NDG hIL15, FcγR KO mice 112653 B-NDG
B-NDG hIL6 mice 110596 B-NDG Promotes human monocyte differentiation
B-NDG hIL7 mice 110597 B-NDG l Promote differentiation of human lymphoid cells
B-NDG hIL7/hIL15 mice 120612 B-NDG Promote differentiation of human T cells and NK cells.
B-NDG hSIRPA mice 110604 B-NDG Maintain progenitor capacity
B-NDG hSIRPA/hCD47 mice 110603 B-NDG Evaluation of pharmacodynamic and toxicity of CD47, SIRPA targeted drugs
B-NDG hTHPO mice 110590 B-NDG Without irradiation, it reduces the damage caused by irradiation to mice
B-NDG hairless mice 110598 B-NDG l Hairless, easy to measure tumor
B-NDG nude mice 110587 B-NDG Human immune system reconstitution
B-Rag2 KO mice 110809 C57BL/6N l No mature T or B cellsl Used for efficacy and toxicity verification
B-hCD98HC/Rag2 KO mice 112326 C57BL/6N Used for verification of efficacy and toxicity of drugs targeting human CD98HC
B-hTFR1/Rag2 KO mice 112904 C57BL/6
B-nude rats 112457 Sprague Dawley
hPBMC-B-NDG mice 112469 NOD scid Human T cell reconstitution, Human-derived cell or tissue engraftment, Tumor research, Human disease model study, Efficacy and toxicity verification for human targeted drugs
huHSC-B-NDG HLA-A2.1 mice 112865 B-NDG
huHSC-B-NDG MGMT3 mice 112479 B-NDG
huHSC-B-NDG hCSF1/hTHPO mice 112863 B-NDG
huHSC-B-NDG hIL15 mice 112478 B-NDG
huHSC-B-NDG hIL6 mice 112864 B-NDG
huHSC-B-NDG hTHPO mice 112480 B-NDG
huHSC-B-NDG mice 112470 NOD scid Human immune system reconstitution, mainly T cells and B cells, reconstitution Human-derived cell or tissue engraftment Tumor research, Human disease model study, Efficacy and toxicity verification
huNK-B-NDG hIL15 mice 112482 B-NDG
huPBMC-B-NDG B2m KO mice plus 112484 B-NDG
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