Immunodeficient (B-NDG) Mice

Humanized immunodeficient mice offer a versatile model for the assessment of human-based immunotherapies. Our team at Biocytogen independently designed and generated the severely immunodeficient B-NDG mouse model (NOD-Prkdc^scidIL2rg^tm1/Bcgen). By deleting the IL2rg gene from the NOD-scid genetic background, the resulting B-NDG mice lack mature T cells, B cells, functional NK cells, and display cytokine signaling deficiencies. These collective properties render B-NDG mice with the highest degree of immunodeficiency, making B-NDG mice most suitable for engraftment and growth of human hematopoietic stem cells (HSCs), peripheral blood mononuclear cells (PBMCs), and human tumor cells/tissues. 

• Prkdc null mice (scid): deficient in functional T cells and B cells, as well as an absence of lymphocytes, altogether recapitulating the severe combined immunodeficiency (scid) phenotype seen in human patients. 
• NOD-scid (or NSG) mice: NOD mice combined with Prkdc gene knockout results in a loss of functional T cells, B cells and the complement system, in addition to reducing the activity of NK cells. 
• IL2rg null mice: disruption of the IL2rg gene leads to impaired immune function, characterized by reduced T and B cells, and loss of NK cells. 

Advantages of B-NDG Mice

• Model currently holds the highest degree of immunodeficiency among all immunodeficiency models
• Longer lifespan than NOD-scid or NSG mice; 1.5 years on average
• Minimal to absent rejection of human-derived cells and tissues
• More efficient for CDX model generation
• No B lymphocyte leakage

Major Applications

• Human-derived cell or tissue engraftment
• Tumor and tumor stem cell research
• ES and iPS cell research
• Hematopoiesis and immunology studies
• Human infectious disease studies
• Development of humanized models