Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hITGA4 mice.
The exons 2-27 of mouse Itga4 gene that encode the extracellular domain were replaced by human ITGA4 exons 2-27 in B-hITGA4 mice.
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mRNA expression analysis
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Strain specific analysis of ITGA4 gene expression in wild-type mice and homozygous B-hITGA4 mice by RT-PCR.
Mouse Itga4 mRNA was detectable in splenocytes of wild-type mice(+/+). Human ITGA4 mRNA was detectable in homozygous B-hITGA4 mice(H/H) .
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Protein expression analysis
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DCs
Strain specific ITGA4 expression analysis in homozygous B-hITGA4 mice by flow cytometry
Splenocytes were collected from wild-type C57BL/6 mice(+/+) and homozygous B-hITGA4 mice(H/H). Mouse ITGA4 was exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 was exclusively detectable in homozygous B-hITGA4 mice.
NK cells
Strain specific ITGA4 expression analysis in homozygous B-hITGA4 mice by flow cytometry.
Splenocytes were collected from wild-type C57BL/6 mice(+/+) and homozygous B-hITGA4 mice(H/H). Mouse ITGA4 was exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 was exclusively detectable in homozygous B-hITGA4 mice.
Monocytes
Strain specific ITGA4 expression analysis in homozygous B-hITGA4 mice by flow cytometry.
Splenocytes were collected from wild-type C57BL/6 mice(+/+) and homozygous B-hITGA4 mice(H/H). Mouse ITGA4 was exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 was exclusively detectable in homozygous B-hITGA4 mice.
Macrophages
Strain specific ITGA4 expression analysis in homozygous B-hITGA4 mice by flow cytometry.
Splenocytes were collected from wild-type C57BL/6 mice(+/+) and homozygous B-hITGA4 mice(H/H). Mouse ITGA4 was exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 was exclusively detectable in homozygous B-hITGA4 mice.
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Summary
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mRNA expression analysis
Mouse Itga4 mRNA was detectable in splenocytes of wild-type mice(+/+). Human ITGA4 mRNA was detectable in homozygous B-hITGA4 mice(H/H).
Protein expression analysis
Mouse ITGA4 was exclusively detectable in wild-type C57BL/6 mice. Human ITGA4 was exclusively detectable in homozygous B-hITGA4 mice.
