Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCD98/Rag2 KO mice.
The exons 2-10 of mouse Cd98 gene that encode the extracellular domain were replaced by human CD98 exons 4-12 in B-hCD98/Rag2 KO mice. The exon 3 encoding the full coding sequence and 3’UTR region of mouse Rag2 gene were knocked out in B-hCD98/Rag2 KO mice, resulting in a disruption of the Rag2 gene.
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Protein expression analysis in brain endothelial cells
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Strain specific CD98 expression analysis in homozygous B-hCD98/Rag2 KO mice by flow cytometry.
Brain were collected from wild type (WT) mice (+/+) and homozygous B-hCD98/Rag2 KO mice (H/H;-/-), and analyzed by flow cytometry with species-specific anti-CD98 antibody after trypsin digestion. Mouse CD98 was detectable in WT mice (+/+). Human CD98 was exclusively detectable in homozygous B-hCD98/Rag2 KO mice (H/H;-/-) but not in WT mice (+/+).
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Protein expression analysis in T cells
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Strain specific CD98 expression analysis in homozygous B-hCD98/Rag2 KO mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD98/Rag2 KO mice (H/H;-/-), and analyzed by flow cytometry with species-specific anti-CD98 antibody. Mouse CD98 was detectable in WT mice (+/+) and human CD98 was not detectable in WT mice (+/+). There were no T cells in homozygous B-hCD98/Rag2 KO mice.
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Protein expression analysis in B cells
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Strain specific CD98 expression analysis in homozygous B-hCD98/Rag2 KO mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD98/Rag2 KO mice (H/H;-/-), and analyzed by flow cytometry with species-specific anti-CD98 antibody. Mouse CD98 was detectable in WT mice (+/+) and human CD98 was not detectable in WT mice (+/+). There were no B cells in homozygous B-hCD98/Rag2 KO mice.
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Protein expression analysis in NK cells
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Strain specific CD98 expression analysis in homozygous B-hCD98/Rag2 KO mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD98/Rag2 KO mice (H/H;-/-), and analyzed by flow cytometry with species-specific anti-CD98 antibody. Mouse CD98 was detectable in WT mice (+/+). Human CD98 was exclusively detectable in homozygous B-hCD98/Rag2 KO mice (H/H;-/-) but not in WT mice (+/+).
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Summary
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Protein expression analysis:
Human CD98 was exclusively detectable on brain endothelial cells and NK cells of homozygous B-hCD98/Rag2 KO mice (H/H;-/-) but not in WT mice (+/+). There were no T and B cells in homozygous B-hCD98/Rag2 KO mice relative to WT mice (+/+).
Mouse CD98 was detectable on brain endothelial cells, T cells, B cells and NK cells in WT mice (+/+).
