Basic Information
Description
The exogenous promoter and mouse Cd30 coding sequence were inserted into the mouse genome randomly. Mouse Cd30 is highly expressed on the surface of B-Tg(mCd30) MC38 cells.
-
Targeting strategy
-
Gene targeting strategy for B-Tg(mCd30) MC38 cells. The exogenous promoter and mouse Cd30 coding sequence were inserted into the mouse genome randomly.
-
Protein Expression Analysis
-
Cd30 expression analysis in B-Tg(mCd30) MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-Tg(mCd30) MC38 cultures were stained with species-specific anti-Cd30 antibody. Mouse Cd30 was detected on the surface of B-Tg(mCd30) MC38 cells but not wild-type MC38 cells. The 1-B03 clone of B-Tg(mCd30) MC38 cells was used for in vivo experiments.
-
Tumor growth curve & Body weight changes
-
Subcutaneous homograft tumor growth of B-Tg(mCd30) MC38 cells. B-Tg(mCd30) MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6N mice (female, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-Tg(mCd30) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
