Basic Information
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Targeting strategy
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Gene targeting strategy for B-hEBI3 mice.
The exons 1-5 of mouse Ebi3 gene that encode the full-length protein were replaced by human EBI3 exons 1-5 in B-hEBI3 mice.
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mRNA and protein expression analysis
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Strain specific analysis of EBI3 gene expression in wild-type mice and B-hEBI3 mice by RT-PCR. Mouse Ebi3 mRNA was detectable in splenocytes of wild-type mice (+/+). Human EBI3 mRNA was detectable only in homozygous B-hEBI3 mice (H/H) but not in wild-type mice.
Strain specific EBI3 expression analysis in homozygous B-hEBI3 mice by western blot. Spleen, Kidney, small intestine and muscle tissue were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hEBI3 mice (H/H), and analyzed by western blot with anti-EBI3 antibody. Human EBI3 was detectable in wild-type mice and homozygous B-hEBI3 mice, as the antibody is crossly reactive with EBI3 in human and mice.
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Summary
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mRNA and protein expression analysis:
Human EBI3 mRNA was detectable in B-hEBI3 mice, and we proved that the protein could be expressed normally by western blot analysis.
