Gene Editing Service Technologies

Our innovative gene-editing technology increases gene-editing efficiency by 10 to 20 fold,
making our custom model development process faster and more affordable for your research.

Site-specific gene modification usually uses homologous recombination of endogenous DNA with exogenous DNA. Homologous recombination is a natural genetic recombination event in which specific nucleotide sequences are randomly exchanged between similar or identical residues.

In advancing genetic engineering, we as scientists have learned to utilize the phenomena of homologous recombination to modify or gene-edit mouse embryonic stem cells (ESC). Gene targeting technology has evolved with the emergence of DNA nuclease-based gene-editing, including Zinc Finger Nuclease (ZFN), and more recent CRISPR/Cas9 technology, which allows even more precise and intentional genetic changes. Compared to ZFN and TALEN, CRISPR/Cas9 technology is more efficient, shortens the timeline/reduces the cost of production, breaks species constraints, and has the potential to directly edit genes in patient tissues.

Due to the typical results of experimentally low rates of homologous recombination, Biocytogen developed an innovative gene targeting technology: the CRISPR/Cas9-based Extreme Genome Editing — EGE™ — system, which can knock in large DNA fragments (> 5 kb) in the genomes of mice, rats and cell lines up-to 20-fold more efficiently than conventional CRISPR/Cas9 methods. EGE™ is successful for 98% of projects, which minimizes the timeline to generate genetically engineered animal and cell models.

Technology Advantages & Disadvantages Applications Timeline
CRISPR/EGE™-based Gene Editing
  • High efficiency
  • Large gene knockout/knockin capabilities
  • Simultaneously targets multiple genes
  • Applicable to many species
  • Easy to construct
  • Off-target effects can be reduced by choosing the appropriate sgRNA and eliminated by breeding; southern blot is used to screen out random insertions
Conventional KO mice/rats 5-7 months
Conditional KO mice/rats
ROSA26 locus gene KI mice/rats
Gene KI/ Mutation mice/rats
Humanized mice/rats
Reporter/KO-then-cKO mice/rats
Normal cell lines KO/KI
hESC/iPS cells KO/KI
ESC/HR-based Gene Editing
  • Accurate, precise and reliably established
  • The only targeting technology which can meet the requirements of almost all genome modifications
  • Because ES cells are required, only mouse cells can be used for this technique
  • Time consuming
  • Heavy workload
  • High cost
Conventional knockout (KO) mice 7-11 months
Conditional KO mice
ROSA26 locus gene knockin (KI) mice
Gene KI/ Mutation mice
Humanized mice
Reporter/KO-then-cKO mice
Transgenic Technology
  • Fast timeline
  • Low cost
  • Super large fragment insertion
  • Disadvantages include random insertion, instability, large number of founders that can cost more time and money
Transgenic mice/rats 2-3 months for F0
Chromosome Engineering
  • Gene cluster replacement at the megabase scale
  • A unique technology for specific applications such as antibody gene cluster humanization
Mouse Upon request
Other Services
  • In addition to full-scale gene editing services, Biocytogen also provides milestone services related to gene editing
CRISPR plasmid construction; CRISPR activity assay; Donor plasmid construction Upon request


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