AACR 2023: B-hCD94/hNKG2A Mice Provide a Preclinical Tool for Evaluating Therapeutic Antibodies Targeting Human NKG2A
Used in this poster: B-hCD94/hNKG2A mice
Conclusions
- mRNA expression analysis:
Mouse Cd94 and Nkg2a mRNAs were detected in splenocytes isolated from wild-type mice, while human CD94 and NKG2A mRNAs were detected in homozygous B-hCD94/hNKG2A mice. - Protein expression analysis:
Human CD94 and NKG2A proteins were detected in homozygous B-hCD94/hNKG2A mice, but not in wild-type mice. - Immune cell analysis:
CD94 and NKG2A humanization does not change the overall development, differentiation or distribution of spleen, lymph node (data not shown), and blood (data not shown) immune cells in B-hCD94/hNKG2A mice. - In vivo efficacy of an anti-human NKG2A antibody:
Anti-human NKG2A mono- and combination therapy controlled B-hHLA-E MC38 tumor growth in B-hCD94/hNKG2A mice.
