Humanized ICOS Mice as a Novel Tool for Predicting and Monitoring T-cell-mediated Immunotherapy Response
SUMMARY
mRNA expression analysis:
Mouse Icos mRNA was detected in thymic tissue isolated from wild-type and heterozygous B-hICOS (H/+) mice. Human ICOS mRNA was detected only in heterozygous B-hICOS (H/+) mice. The positive band was confirmed by sequencing.
Protein expression analysis:
Human ICOS was exclusively detected in homozygous B-hICOS (H/H) mice.
Proliferation analysis:
CD4+ and CD8+ T cell activation in heterozygous B-hICOS mice (H/+) and homozygous B-hICOS mice (H/H) was upregulated by anti-mCD3ε and human ICOSL recombinant protein, similar to the activation by anti-mCD3ε and anti-mCD28. This demonstrates that the introduction of human ICOS in place of its mouse counterpart does not affect T cell activation.
Functional assay:
The basal serum levels of IgG1 in B-hICOS mice were significantly higher than those in wild-type mice, which may be caused by the humanization of ICOS. OVA-specific IgG1 and IgE levels in B-hICOS mice were similar to those in wild-type mice.
Used in the study: B-hICOS mice
