Basic Information
-
Targeting strategy
-
Gene targeting strategy for B-NDG hB2M/HLA-A11.1 mice. The B2m gene (Exon1 to Exon3) of mouse was replaced by the sequence encompassing the human B2M CDS and HLA-A11.1 gene.
-
Protein expression analysis
-
B2M and HLA-A11.1 expression analysis in homozygous B-NDG hB2M/HLA-A11.1 mice by flow cytometry. Splenocytes were collected from B-NDG mice (+/+) and homozygous B-NDG hB2M/HLA-A11.1 mice (H/H), and analyzed by flow cytometry with strain specific antibodies. Mouse B2M was detectable in B-NDG mice but not in B-NDG hB2M/HLA-A11.1 mice. Human B2M and HLA-ABC were exclusively detectable in homozygous B-NDG hB2M/HLA-A11.1 mice but not in B-NDG mice.
-
Summary
-
Protein expression analysis:
Mouse B2M was detectable in B-NDG mice but not in B-NDG hB2M/HLA-A11.1 mice. Human B2M and HLA-ABC were exclusively detectable in homozygous B-NDG hB2M/HLA-A11.1 mice but not in B-NDG mice.
