Basic Information
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Targeting strategy
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Gene targeting strategy for B-NDG hB2M/HLA-A3.1 mice. The B2m gene (Exon1 to Exon3) of mouse was replaced by the sequence encompassing the human B2M CDS and HLA-A3.1 gene.
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Protein expression analysis
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B2M and HLA-A3.1 expression analysis in homozygous B-NDG hB2M/HLA-A3.1 mice by flow cytometry. Splenocytes were collected from B-NDG mice (+/+) and homozygous B-NDG hB2M/HLA-A3.1 mice (H/H), and analyzed by flow cytometry with strain specific antibodies. Mouse B2M was detectable in B-NDG mice but not in B-NDG hB2M/HLA-A3.1 mice. Human B2M and HLA-A3 were exclusively detectable in homozygous B-NDG hB2M/HLA-A3.1 mice but not in B-NDG mice.
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Summary
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Protein expression analysis:
Mouse B2M was detectable in B-NDG mice but not in B-NDG hB2M/HLA-A3.1 mice. Human B2M and HLA-A3 were exclusively detectable in homozygous B-NDG hB2M/HLA-A3.1 mice but not in B-NDG mice.
