Basic Information

Strain Name
NOD.CB17-PrkdcscidBcgenIl2rgtm1BcgenThpotm1(THPO)Bcgen/Bcgen
Common Name
huHSC-B-NDG hTHPO mice
Background
B-NDG
Catalog Number
112480
NCBI Gene ID
Related Genes
MGDF, MKCSF, ML, MPLLG, THCYT1, TPO

B-NDG hTHPO mice are well suited for human immune system engraftment

B-NDG hTHPO mice

Human CD34+ HSCs (5E5) were intravenous engrafted into wild-type B-NDG mice and homozygous B-NDG hTHPO mice (female, 6 week-old, n=20). B-NDG mice were treated with 2Gy-irradiation. B-NDG hTHPO mice were not irradiated. Flow cytometric analysis of peripheral blood lymphocyte from the two mice after engraftment with human CD34+ HSCs were shown in the figure above. Results showed that the survival rate of B-NDG hTHPO mice was higher than that of B-NDG mice. The percentages of human CD45+ leukocytes, T cells, B cells, NK cells, myeloid cells, monocytes, macrophages and neutrophils in B-NDG hTHPO mice were similar to those in B-NDG mice, demonstrating that the humanized mouse model engrafted with human CD34+ HSCs was successfully constructed. Values are expressed as mean ± SEM. HSCs: hematopoietic stem cells.

Back to top