B-hROR1 MC38

Basic Information

Common Name
B-hROR1 MC38
Aliases
NTRKR1, dJ537F10.1
Catalog Number
310807
Cell Type/ Disease
Colon Carcinoma
Species/ Strain
Mus musculus, C57BL/6
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Description

The mouse Ror1 gene was replaced by human ROR1 coding sequence in B-hROR1 MC38 cells. Human ROR1 is highly expressed on the surface of B-hROR1 MC38 cells.

Targeting strategy

The exogenous promoter and human ROR1 coding sequence was inserted to replace part of murine exon 3 and all of exon 4. The insertion disrupts the endogenous murine Ror1 gene, resulting in a non-functional transcript.

Protein expression analysis

ROR1 expression analysis in B-hROR1 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hROR1 MC38 cultures were stained with species-specific anti-ROR1 antibody. Human ROR1 was detected on the surface of B-hROR1 MC38 cells but not wild-type MC38 cells. The 1-G06 clone of B-hROR1 MC38 cells was used for in vivo experiments.

Tumor growth curve

Subcutaneous homograft tumor growth of B-hROR1 MC38 cells. B-hROR1 MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6N mice (female, 8-week-old, n=8). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hROR1 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

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