A CAG-iCre-PolyA sequence cassette was inserted into the rat ROSAS26 locus. This strain was maintained on a Sprague Dawley genetic background.
Primer Sequences and Predicted Product Size
Primer set: 211350-WT-F/211350-WT-R
Primer set: 211350-WT-F/211350-Mut-R
Gel image of PCR products. PCR products are separated by gel electrophoresis on a 2% agarose gel. PC: samples used for PCR were collected from heterozygous B-CAG-iCre rats; WT: samples used for PCR were collected from wild-type rats; H2O: blank control.