ROSA26 gene produces a long non-coding RNA (lncRNA) that is under the control of a constitutive promoter. This locus is a widely used site for the integration of transgenes and reporter constructs.
T cells were isolated from spleen in single positive mice and double-positive mice.The proportion of lymphocyte subpopulation were analyzed by flow cytometry.
Results: tdTomato+ cells were detected in the double-positive mice
Protein expression analysis (peripheral blood )
T cells were isolated from peripheral blood in the single positive mice and the double- positive mice. The proportion of lymphocyte subpopulation were analyzed by flow cytometry.
Results: tdTomato+ cells were detected in the double-positive mice.
Primer Sequence (5’-3’) Tm
ROSA-GT-F AGTCGCTCTGAGTTGTTATCAG 55 WT:469
ROSA-GT-R TGAGCATGTCTTTAATCTACCTCGATG 57 ROSA-GT-F AGTCGCTCTGAGTTGTTATCAG 55 Mut:268 ROSA-PCR-R AGTCCCTATTGGCGTTACTATGG 57
1. Srinivas, Shankar, et al. "Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus." BMC developmental biology 1.1 (2001): 4.