The Dmp1 gene encodes the dentin matrix acidic phosphoprotein 1, an extracellular matrix protein and a member of the small integrin-binding ligand N-linked glycoprotein family. This protein is critical for proper mineralization of bone and dentin. In this strain, Cre recombinase expression is under the control of Dmp1 promoter in a bone and tooth tissue pattern. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in Dmp1 expressing cells.
Primer Sequence (5’-3’) Tm
Dmp1iCre primer 1 TGGAAGCTGACAGTAGGAAAC 56 WT: 334 Dmp1iCre primer 2 TGACATCATCCCACGTACTTAAGC 58 Dmp1iCre primer 1 TGGAAGCTGACAGTAGGAAAC 56 Mut: 208 Dmp1iCre primer 3 TGGTGCACAGTCAGCAGGTTG 61
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