Basic Information
- Function research of genes
- This Drd2Cre model is an efficient tool to study various gene functions when crossed with rats with different loxP site-flanked genes of interest, especially in studies of D2 dopamine receptor subtype-expressing neurons, and may be useful for studying dopamine-sensitive behaviors such as addiction.
Description
The Drd2 gene encodes dopamine receptor D2. In this strain, Cre recombinase expression is under the control of Drd2 promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in Drd2 expressing cells.
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Targeting Strategy
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A P2A-NLS-Cre sequence cassette was placed between the coding sequence of exon 3 and 3’UTR of the Drd2 gene. This strain was maintained on a Sprague Dawley genetic background.
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Phenotype Analysis
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k Immunofluorescent images of tdTomato and DRD2 in the striatum of
Drd2 reporter rats. Arrows indicate the colocalization of tdTomato and
DRD2. Scale bar, 25 µm. l Western blot of DRD2 and GAPDH in the
striatum from Drd2::Cre+/− and WT rats. Left, representative blots, right,
quantifcation results. NS not signifcant, n= 3, t test
(source: Yu Q et al.,2019) -
Reference
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- Yu Q, Liu YZ, Zhu YB, Wang YY, Li Q, Yin DM. Genetic labeling reveals temporal and spatial expression pattern of D2 dopamine receptor in rat forebrain. Brain Struct Funct. 2019 Apr;224(3):1035-1049. [PubMed: 30604007]
- Bunzow, J. R., Van Tol, H. H., Grandy, D. K., Albert, P., Salon, J., Christie, M., Machida, C. A., Neve, K. A., Civelli, O. Cloning and expression of a rat D2 dopamine receptor cDNA. Nature 336: 783-787, 1988. [PubMed: 2974511]
- Duan, J., Wainwright, M. S., Comeron, J. M., Saitou, N., Sanders, A. R., Gelernter, J., Gejman, P. V. Synonymous mutations in the human dopamine receptor D2 (DRD2) affect mRNA stability and synthesis of the receptor. Hum. Molec. Genet. 12: 205-216, 2003. [PubMed: 12554675]
- Basu, S., Nagy, J. A., Pal, S., Vasile, E., Eckelhoefer, I. A., Bliss, V. S., Manseau, E. J., Dasgupta, P. S., Dvorak, H. F., Mukhopadhyay, D. The neurotransmitter dopamine inhibits angiogenesis induced by vascular permeability factor/vascular endothelial growth factor. Nature Med. 7: 569-574, 2001. [PubMed: 11329058]
- Balk, J.-H., Picetti, R., Salardi, A., Thirlet, G., Dierich, A., Depaulls, A., Le Meur, M., Borrelli, E. Parkinsonian-like locomotor impairment in mice lacking dopamine D2 receptors. Nature 377: 424-428, 1995. [PubMed: 7566118]
