Basic Information

Strain Name
SD-Drd2 tm1(Cre) /Bcgen
Stock Number
210488
Common Name
B-Drd2-Cre rats
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Gene symbol and name
Drd2 (dopamine receptor D2); D2R; Drd-2
Background
SD
NCBI Gene ID
Species
Sprague Dawley
Appearance
White
Genotypes
Drd2 Cre (wt/mut)
Application
  • Function research of genes
  • This Drd2Cre model is an efficient tool to study various gene functions when crossed with rats with different loxP site-flanked genes of interest, especially in studies of D2 dopamine receptor subtype-expressing neurons, and may be useful for studying dopamine-sensitive behaviors such as addiction.
Development
A P2A-NLS-Cre sequence cassette was placed between the coding sequence of exon 3 and 3’UTR of the Drd2 gene. This strain was maintained on a Sprague Dawley genetic background.

Description

The Drd2 gene encodes dopamine receptor D2. In this strain, Cre recombinase expression is under the control of Drd2 promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in Drd2 expressing cells.

Targeting Strategy

A P2A-NLS-Cre sequence cassette was placed between the coding sequence of exon 3 and 3’UTR of the Drd2 gene. This strain was maintained on a Sprague Dawley genetic background.

Phenotype Analysis

k Immunofluorescent images of tdTomato and DRD2 in the striatum of
Drd2 reporter rats. Arrows indicate the colocalization of tdTomato and
DRD2. Scale bar, 25 µm. l Western blot of DRD2 and GAPDH in the
striatum from Drd2::Cre+/− and WT rats. Left, representative blots, right,
quantifcation results. NS not signifcant, n= 3, t test
(source: Yu Q et al.,2019)

Reference

  1. Yu Q, Liu YZ, Zhu YB, Wang YY, Li Q, Yin DM. Genetic labeling reveals temporal and spatial expression pattern of D2 dopamine receptor in rat forebrain. Brain Struct Funct. 2019 Apr;224(3):1035-1049. [PubMed: 30604007]
  2. Bunzow, J. R., Van Tol, H. H., Grandy, D. K., Albert, P., Salon, J., Christie, M., Machida, C. A., Neve, K. A., Civelli, O. Cloning and expression of a rat D2 dopamine receptor cDNA. Nature 336: 783-787, 1988. [PubMed: 2974511]
  3. Duan, J., Wainwright, M. S., Comeron, J. M., Saitou, N., Sanders, A. R., Gelernter, J., Gejman, P. V. Synonymous mutations in the human dopamine receptor D2 (DRD2) affect mRNA stability and synthesis of the receptor. Hum. Molec. Genet. 12: 205-216, 2003. [PubMed: 12554675]
  4. Basu, S., Nagy, J. A., Pal, S., Vasile, E., Eckelhoefer, I. A., Bliss, V. S., Manseau, E. J., Dasgupta, P. S., Dvorak, H. F., Mukhopadhyay, D. The neurotransmitter dopamine inhibits angiogenesis induced by vascular permeability factor/vascular endothelial growth factor. Nature Med. 7: 569-574, 2001. [PubMed: 11329058]
  5. Balk, J.-H., Picetti, R., Salardi, A., Thirlet, G., Dierich, A., Depaulls, A., Le Meur, M., Borrelli, E. Parkinsonian-like locomotor impairment in mice lacking dopamine D2 receptors. Nature 377: 424-428, 1995. [PubMed: 7566118]
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