Basic Information

Strain Name
C57BL/6-Prrx1tm1(icre)Bcgen/Bcgen
Stock Number
110130
Common Name
B-Prrx1-iCre mice
Related Genes
Prrx1 (paired related homeobox 1); K-2; Pmx; Pmx1; Prx1; mHox; AA755424; AI385634; AI843499; A230024N07Rik
Background
C57BL/6
Appearance
Black
Development

In this strain, Cre recombinase expression is under the control of Prrx1 promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in Prrx1 expressing cells.

NCBI Gene ID

Targeting Strategy

An F2A-iCre sequence cassette was placed between the coding sequence of exon 1 and 5’UTR of the Prrx1 gene in C57BL/6 ES cells. This strain was maintained on a C57BL/6 genetic background.

Details

Genotyping Information

Primer Sequence (5’-3’) Tm
(℃)
Product size
(bp)
Prrx1 iCre primer 1 AGCGTTTGGTGTTGATTCGAGC 60 WT: 267
Prrx1 iCre primer 2 AGTCCCGGTGACTCCAGCAG 62
Prrx1 iCre primer 1 AGCGTTTGGTGTTGATTCGAGC 62 Mut: 108
Prrx1 iCre primer 3 TGGTGCACAGTCAGCAGGTTG 61

Polymerase: Taq

 

References

1. Durland JL; Sferlazzo M; Logan M; Burke AC. 2008. Visualizing the lateral somitic frontier
in the Prx1Cre transgenic mouse. J Anat 212(5):590-602. [PubMed: 18430087] [MGI Ref ID J:209265]
2. Seo HS; Serra R. 2007. Deletion of Tgfbr2 in Prx1-cre expressing mesenchyme results in
defects in development of the long bones and joints. Dev Biol 310(2):304-16. [PubMed:
17822689] [MGI Ref ID J:128010]

Publications

Fuling Zhou, Xiaoyan Liu, Jinxian Wu et al. Mesenchymal stem cell-derived S100A8 facilitates leukemia stem cell maintenance via TLR4/PI3K/Akt signaling, 13 May 2024, PREPRINT (Version 1) available at Research Square

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