Basic Information
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Protein Expression Analysis
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Species-specific CD3E expression analysis in wild-type BALB/c mice and homozygous humanized B-hCD3E/h4-1BB mice(C) by flow cytometry. Splenocytes were collected from wild-type BALB/c mice (+/+) and homozygous B-hCD3E/h4-1BB mice(C) (H/H; H/H), and analyzed by flow cytometry. Mouse CD3E was only detectable in wild-type BALB/c mice. Human CD3E was exclusively detected in homozygous B-hCD3E/h4-1BB mice(C), but not in wild-type BALB/c mice.
Species-specific 4-1BB expression analysis in wild-type BALB/c mice and homozygous humanized B-hCD3E/h4-1BB mice(C) by flow cytometry. Splenocytes were collected from wild-type BALB/c mice (+/+) and homozygous B-hCD3E/h4-1BB mice(C) (H/H; H/H) stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry. Mouse 4-1BB was only detected in wild-type BALB/c mice. Human h4-1BB was exclusively detected in homozygous B-hCD3E/h4-1BB mice(C), but not in wild-type BALB/c mice.
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Summary
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Protein expression analysis:
Mouse CD3E and 4-1BB were only detectable on T cells from spleen of wild-type BALB/c mice. Human CD3E and 4-1BB were only detectable on T cells from spleen of homozygous B-hCD3E/h4-1BB mice(C) but not in wild-type mice.
