Basic Information
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Protein expression analysis of CD19
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Strain specific CD19 expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hCD3EDG/hCD19 mice by flow cytometry. Splenocytes and blood cells were collected from wild-type C57BL/6 mice and homozygous B-hCD3EDG/hCD19 mice (female, 7-week-old, n=1). Protein expression was analyzed with anti-mouse CD19 antibody (Biolegend, 115507) and anti-human CD19 antibody (Biolegend, 392503) by flow cytometry. Mouse CD19 was only detectable in wild-type C57BL/6 mice. Human CD19 was exclusively detectable in homozygous B-hCD3EDG/hCD19 mice, but not in wild-type C57BL/6 mice.
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Protein expression analysis of CD3E
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Strain specific CD3E expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hCD3EDG/hCD19 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice and homozygous B-hCD3EDG/hCD19 mice (female, 7-week-old, n=1). Protein expression was analyzed with anti-mouse CD3E antibody (Biolegend, 100312) and anti-human CD3E antibody (BD Horizon™, 562426) by flow cytometry. Mouse CD3E was only detectable in wild-type C57BL/6 mice. Human CD3E was exclusively detectable in homozygous B-hCD3EDG/hCD19 mice, but not in wild-type C57BL/6 mice.
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Frequency of leukocyte subpopulations in spleen
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Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6 mice and homozygous B-hCD3EDG/hCD19 mice (female, 8-week-old, n=3). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, dendritic cells, monocytes, macrophages, neutrophils, CD4+ T cells, CD8+ T cells and Tregs in B-hCD3EDG/hCD19 mice were similar to those in C57BL/6 mice, demonstrating that humanization of CD3EDG and CD19 do not change the frequency or distribution of these cell types in spleen. Values are expressed as mean ± SEM.
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Frequency of leukocyte subpopulations in blood
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Frequency of leukocyte subpopulations in spleen by flow cytometry. Blood cells were isolated from wild-type C57BL/6 mice and homozygous B-hCD3EDG/hCD19 mice (female, 8-week-old, n=3). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, dendritic cells, monocytes, macrophages, neutrophils, CD4+ T cells, CD8+ T cells and Tregs in B-hCD3EDG/hCD19 mice were similar to those in C57BL/6 mice, demonstrating that humanization of CD3EDG and CD19 do not change the frequency or distribution of these cell types in blood. Values are expressed as mean ± SEM.
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Frequency of leukocyte subpopulations in lymph node
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Frequency of leukocyte subpopulations in spleen by flow cytometry. Lymph nodes were isolated from wild-type C57BL/6 mice and homozygous B-hCD3EDG/hCD19 mice (female, 8-week-old, n=3). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, CD4+ T cells, CD8+ T cells and Tregs in B-hCD3EDG/hCD19 mice were similar to those in C57BL/6 mice, demonstrating that humanization of CD3EDG and CD19 do not change the frequency or distribution of these cell types in lymph nodes. Values are expressed as mean ± SEM.
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Frequency of leukocyte subpopulations in thymus
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Frequency of leukocyte subpopulations in spleen by flow cytometry. Thymocytes were isolated from wild-type C57BL/6 mice and homozygous B-hCD3EDG/hCD19 mice (female, 8-week-old, n=3). A. Frequency of T cell subpopulations. Percentages of CD4+ T cells, CD8+ T cells and Tregs in B-hCD3EDG/hCD19 mice were similar to those in C57BL/6 mice, demonstrating that humanization of CD3EDG and CD19 do not change the frequency or distribution of these cell types in thymus. Values are expressed as mean ± SEM.