Basic Information
Strain Name
SD-Ldlrtm1/Bcgen
Stock Number
210506
Common Name
B-Ldlr KO rats
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
Ldlr (low density lipoprotein receptor)
Background
Sprague Dawley
Appearance
White
Genotypes
LdlrKO (mut/mut)
NCBI Gene ID
Description
Ldlr KO rats were developed using targeted TALEN technology in the Sprague-Dawley strain. This model possesses a 2 bp deletion within Exon 1 of Ldlr on chromosome 8. This strain was maintained by mating homozygous males with homozygous females on a Sprague-Dawley genetic background.
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Targeting Strategy
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Model validation
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Homozygous Ldlrtm1/Bcgen rats demonstrate significantly elevated serum cholesterol levels at 4 months of age. -
Details
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Genotyping Information
Primer Sequence (5’-3’) Tm (℃)
Product size (bp) LdlrKO primer 1 GTAGTCAGTCACCAGTTGACCCAGT 62 Mut: 875 WT: 877
LdlrKO primer 2 CAGAGGCACAACTATGAAGCTGACC 62 Polymerase: Taq
The fragments need to be verified by sequencing.
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References
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- Afek A; Keren G; Harats D; George J. 2001. Whole body hyperthermia accelerates atherogenesis in low-density lipoprotein receptor deficient mice. Exp Mol Pathol 71(1):63-72. [PubMed: 11502098]
- Adachi H; Kondo T; Koh GY; Nagy A; Oike Y; Araki E. 2011. Angptl4 deficiency decreases serum triglyceride levels in low-density lipoprotein receptor knockout mice and streptozotocin-induced diabetic mice. Biochem Biophys Res Commun 409(2):177-80. [PubMed: 21549101]
- Babaev VR; Ishiguro H; Ding L; Yancey PG; Dove DE; Kovacs WJ; Semenkovich CF; Fazio S; Linton MF. 2007. Macrophage expression of peroxisome proliferator-activated receptor-alpha reduces atherosclerosis in low-density lipoprotein receptor-deficient mice. Circulation 116(12):1404-12. [PubMed: 17724261]
