The recombinant-activating gene 2 (Rag2) plays an important role in the rearrangement and recombination of the genes of immunoglobulin and T cell receptor molecules during the initiation of V(D)J recombination. Loss of Rag2 protein leads to no mature T and B cells, the critical components of the adaptive immune system.
Il2rg is known as the interleukin receptor common gamma chain, which is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21. Mutations in this gene cause X-linked severe combined immunodeficiency.
Primer Sequence(5’-3’) Tm
Product size (bp) Rag2KO primer 1 CCTCGGATTCTCAAAGCAAGGG 62 Mut:881
Rag2KO primer 2 CAGGACGTATGTTACTGGCAAGTG 62 Primer Sequence (5’-3’) Tm
Product size (bp) IL2rgKO primer 1 GCTCCAAGGTCCTCATGTCCAG 62 Mut:915
IL2rgKO primer 2 ATCTTCGCCTTTCTGCCCATGAC 62
The fragments need to be verified by sequencing.
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