B-Rag2/IL2rg KO rats

Basic Information

Description

The B-Rag2-IL2rg knockout rat was developed using targeted TALEN technology in the Sprague-Dawley strain, and this model possesses Rag2 and IL2rg gene deletion. This strain was maintained by mating homozygous males with homozygous females on a Sprague-Dawley genetic background.

Targeting Strategy

 

 

 

 

 

 

 

 

 

 

Details

Genotyping Information

Primer	Sequence(5’-3’)	Tm (℃)	Product size (bp)
Rag2KO primer 1	CCTCGGATTCTCAAAGCAAGGG	62	Mut:881 WT:1002
Rag2KO primer 2	CAGGACGTATGTTACTGGCAAGTG	62

 

Primer	Sequence (5’-3’)	Tm (℃)	Product size (bp)
IL2rgKO primer 1	GCTCCAAGGTCCTCATGTCCAG	62	Mut:915 WT:929
IL2rgKO primer 2	ATCTTCGCCTTTCTGCCCATGAC	62

Polymerase: Taq

The fragments need to be verified by sequencing.

References

1. Corneo, B., et al., Identical mutations in RAG1 or RAG2 genes leading to defective V(D)J recombinase activity can cause either T-B-severe combined immune deficiency or Omenn syndrome. Blood, 2001. 97(9): p. 2772-6. [PubMed: 11313270]
2. Shinkai, Y., et al., RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement. Cell, 1992. 68(5): p. 855-67. [PubMed: 1547487]
3. Rideout, W.M., 3rd, et al., Correction of a genetic defect by nuclear transplantation and combined cell and gene therapy. Cell, 2002. 109(1): p. 17-27. [MGI Ref ID J:11955443]
4. Ohbo, K., et al., Modulation of hematopoiesis in mice with a truncated mutant of the interleukin-2 receptor gamma chain. Blood, 1996. 87(3): p. 956-67. [PubMed: 8562967]
5. Mashimo, T., et al., Generation of knockout rats with X-linked severe combined immunodeficiency (X-SCID) using zinc-finger nucleases. PLoS One, 2010. 5(1): p. e8870. [PubMed: 20111598]
6. Mashimo, T., et al., Generation and characterization of severe combined immunodeficiency rats. Cell Rep, 2012. 2(3): p. 685-94. [PubMed: 22981234]