B-Rag2 KO rats
Bcgen (Beijing Biocytogen Co., Ltd)
Rag2 (recombination activating gene 2)
NCBI Gene ID
Rag2 KO rats were developed using targeted TALEN technology in the Sprague-Dawley strain. This model possesses a 121 bp deletion within Exon 3 of Rag2 on chromosome 3. This strain was maintained by mating homozygous males with homozygous females on a Sprague-Dawley genetic background.
Primer Sequence (5’-3’) Tm
Product size (bp) Rag2KO primer 1 CCTCGGATTCTCAAAGCAAGGG 62 Mut: 881
Rag2KO primer 2 CAGGACGTATGTTACTGGCAAGTG 62
The fragments need to be verified by sequencing.
- Corneo, B., et al., Identical mutations in RAG1 or RAG2 genes leading to defective V(D)J recombinase activity can cause either T-B-severe combined immune deficiency or Omenn syndrome. Blood, 2001. 97(9): p. 2772-6. [PubMed: 11313270]
- Shinkai, Y., et al., RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement. Cell, 1992. 68(5): p. 855-67. [PubMed: 1547487]
- Rideout, W.M., 3rd, et al., Correction of a genetic defect by nuclear transplantation and combined cell and gene therapy. Cell, 2002. 109(1): p. 17-27. [MGI Ref ID J:11955443]