Basic Information

Strain Name
SD-Rag2tm1/Bcgen
Stock Number
210511
Common Name
B-Rag2 KO rats
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
Rag2 (recombination activating gene 2)
Species
Sprague Dawley
Appearance
White
Genotypes
Rag2KO (mut/mut)
NCBI Gene ID

Description

Rag2 KO rats were developed using targeted TALEN technology in the Sprague-Dawley strain. This model possesses a 121 bp deletion within Exon 3 of Rag2 on chromosome 3. This strain was maintained by mating homozygous males with homozygous females on a Sprague-Dawley genetic background.

Details

Genotyping Information

 

Primer Sequence (5’-3’) Tm

()

Product size (bp)
Rag2KO primer 1 CCTCGGATTCTCAAAGCAAGGG 62 Mut: 881

WT: 1002

Rag2KO primer 2 CAGGACGTATGTTACTGGCAAGTG 62

Polymerase: Taq

The fragments need to be verified by sequencing.

References

  1. Corneo, B., et al., Identical mutations in RAG1 or RAG2 genes leading to defective V(D)J recombinase activity can cause either T-B-severe combined immune deficiency or Omenn syndrome. Blood, 2001. 97(9): p. 2772-6. [PubMed: 11313270]
  2. Shinkai, Y., et al., RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement. Cell, 1992. 68(5): p. 855-67. [PubMed: 1547487]
  3. Rideout, W.M., 3rd, et al., Correction of a genetic defect by nuclear transplantation and combined cell and gene therapy. Cell, 2002. 109(1): p. 17-27. [MGI Ref ID J:11955443]