Rag2 KO rats were developed using targeted TALEN technology in the Sprague-Dawley strain. This model possesses a 121 bp deletion within Exon 3 of Rag2 on chromosome 3. This strain was maintained by mating homozygous males with homozygous females on a Sprague-Dawley genetic background.
Primer Sequence (5’-3’) Tm
Product size (bp) Rag2KO primer 1 CCTCGGATTCTCAAAGCAAGGG 62 Mut: 881
Rag2KO primer 2 CAGGACGTATGTTACTGGCAAGTG 62
The fragments need to be verified by sequencing.
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