Tp53tm1/Bcgen rats are a useful model for studying tumor formation, screening in vivo carcinogenicity, and examining the efficacy of therapeutic agents.
The Tp53 KO rat model was developed using targeted TALEN technology in the Sprague-Dawley strain. This model possesses a 12 bp deletion and 2 bp insertion within Exon 2 of Tp53 on chromosome 10. This strain was maintained by mating heterozygous females with homozygous males on a Sprague-Dawley genetic background.
Primer Sequence (5’-3’) Tm
Product size (bp) Tp53KO primer 1 TGGCCGACTTCTTGGTTACTTGT 62 Mut: 1078
Tp53KO primer 2 CTCTGAGGCATAGTCAAAGTCCAC 62
The fragments need to be verified by sequencing.
- Hursting, S.D., et al., Chemoprevention of spontaneous tumorigenesis in p53-knockout mice. Cancer Res, 1995.55(18): p. 3949-53. [PubMed: 7664260]
- van Boxtel, R., et al., Homozygous and heterozygous p53 knockout rats develop metastasizing sarcomas with high frequency. Am J Pathol, 2011. 179(4): p.1616-22. [PubMed: 21854749]
- Hermsen, R., et al., Lack of major genome instability in tumors of p53 null rats. PLoS One, 2015. 10(3): p.e0122066. [PubMed: 25811670]