Basic Information
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Targeting strategy
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Gene targeting strategy for B-hADM mice.
The exons 2-4 of mouse Adm gene that encode the full-length protein were replaced by human ADM exons 2-4 in B-hADM mice. -
Protein expression analysis
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Strain specific ADM expression analysis in homozygous B-hADM mice by ELISA. Plasma was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hADM mice (H/H) and analyzed by ELISA with ADM ELISA kit. ADM was detectable in wild-type mice and homozygous B-hADM mice as the kit is crossly reactive with ADM in human and mice.
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mRNA expression analysis
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Strain specific analysis of ADM mRNA expression in wild-type C57BL/6 mice and B-hADM mice by RT-PCR. Kidney RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hADM mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ADM primers. Mouse Adm mRNA was detectable only in wild-type C57BL/6 mice. Human ADM mRNA was detectable only in homozygous B-hADM mice but not in wild-type mice.
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Summary
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mRNA expression analysis:
Mouse Adm mRNA was detectable in kidney of wild-type C57BL/6 mice. Human ADM mRNA was detectable in B-hADM mice.Protein expression analysis:
ADM was detectable in wild-type mice and homozygous B-hADM mice as the kit is crossly reactive with ADM in human and mice.
