B-hCCL20 mice

Basic Information

Targeting strategy

Gene targeting strategy for B-hCCL20 mice. The full coding region of mouse Ccl20 was replaced by human CCL20 coding region sequences in B-hCCL20 mice.

Protein expression analysis

Species-specific CCL20 protein expression analysis in wild-type and humanized B-hCCL20 mice. Supernatant derived from thymus tissue grinding was collected from wild-type (+/+) and homozygous B-hCCL20 (H/H) mice, and analyzed by ELISA using species-specific CCL20 kits. Murine CCL20 protein was detected in wild-type mice, while human CCL20 protein was exclusively detected in B-hCCL20 mice.

Summary

Protein expression analysis:

Human CCL20 was exclusively detectable in homozygous B-hCCL20 mice but not in wild-type mice.