Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCCL5 mice.
The exons 1-3 of mouse Ccl5 gene that encodes the full-length protein was replaced by human CCL5 exons 1-3 in B-hCCL5 mice. -
Protein expression analysis
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Strain specific CCL5 expression analysis in homozygous B-hCCL5 mice by ELISA. Serum were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCCL5 mice (H/H) and stimulated with 1 mg/mL LPS in vivo for 3 h and analyzed by ELISA with species-specific CCL5 ELISA kit. Mouse CCL5 was detectable in wild-type mice and homozygous B-hCCL5 mice (A). Human CCL5 was detectable in wild-type mice and homozygous B-hCCL5 mice (B).
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mRNA expression analysis
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Strain specific analysis of CCL5 gene expression in wild-type C57BL/6 mice and hCCL5 mice by RT-qPCR. The mRNA expression of hCCL5 in homozygous B-hCCL5 mice (H/H, n=3) was significantly different to those in the wild-type C57BL/6 mice (+/+, n=3).
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Summary
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mRNA expression analysis:
The mRNA expression of CCL5 in homozygous B-hCCL5 mice (H/H, n=3) was significantly different to those in the wild-type C57BL/6 mice (+/+, n=3).
Protein expression analysis:
Human CCL5 was detectable on serum of homozygous B-hCCL5 mice, and mouse CCL5 was detectable in wild-type C57BL/6 mice.
