Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCFB mice.
The exons 1~18 of mouse Cfb gene that encode the full-length protein were replaced by human CFB exons 1~18 in B-hCFB mice. -
Protein expression analysis
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Strain specific hCFB expression analysis in homozygous B-hCFB mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hCFB mice (H/H), and analyzed by ELISA with species-specific CFB ELISA kit. Human CFB was exclusively detectable in homozygous B-hCFB mice but not in wild-type mice.
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mRNA expression analysis
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Strain specific analysis of CFB gene expression in wild-type mice and B-hCFB mice by RT-PCR. Mouse Cfb mRNA was detectable in liver of wild-type mice. Human CFB mRNA was detectable only in homozygous B-hCFB but not in wild-type mice.
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Summary
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mRNA expression analysis:
Mouse Cfb mRNA was exclusively detected in C57BL/6 mice (+/+). Human CFB mRNA was exclusively detected in homozygous B-hCFB mice (H/H), but not in wild-type mice.Protein expression analysis:
The human CFB was exclusively detected in homozygous B-hCFB mice.
