Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCXCL10 mice.
The exons 1~4 of mouse Cxcl10 gene that encode the full-length protein was replaced by human CXCL10 exons 1~4 in B-hCXCL10 mice.
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Protein expression analysis
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Strain specific CXCL10 expression analysis in homozygous B-hCXCL10 mice by ELISA. Serum was isolated from wild-type mice (+/+) and homozygous B-hCXCL10 mice (H/H) and stimulated with 20ug/200ul LPS in vivo, serum was analyzed by ELISA with species-specific CXCL10 ELISA kit. Mouse CXCL10 was detectable in wild-type mice (A). Human CXCL10 was exclusively detectable in homozygous B-hCXCL10 mice but not in wild-type mice (B).
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Summary
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Protein expression analysis:
Mouse CXCL10 was detectable in wild-type mice. Human CXCL10 was exclusively detectable in homozygous B-hCXCL10 mice but not in wild-type mice.
