Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hCXCL7/hCXCL4 mice.
The exons 1-3 of mouse Cxcl7 gene that encode the full-length protein and the exons 1-3 of mouse Cxcl4 gene that encode the full-length protein including the non-coding region between Cxcl7 gene and Cxcl4 gene were replaced by human CXCL7 exons 1-3 and human CXCL4 exons 1-3 in B-hCXCL7/hCXCL4 mice.
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Protein expression analysis
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Strain specific CXCL7 expression analysis in homozygous B-hCXCL7/hCXCL4 mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hCXCL7/hCXCL4 mice (H/H), and analyzed by ELISA with species-specific CXCL7 ELISA kit. Mouse CXCL7 was detectable in wild-type mice. Human CXCL7 was exclusively detectable in homozygous B-hCXCL7/hCXCL4 mice but not in wild-type mice.
Strain specific CXCL4 expression analysis in homozygous B-hCXCL7/hCXCL4 mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hCXCL7/hCXCL4 mice (H/H), and analyzed by ELISA with species-specific CXCL4 ELISA kit. Mouse CXCL4 was detectable in wild-type mice. Human CXCL4 was exclusively detectable in homozygous B-hCXCL7/hCXCL4 mice but not in wild-type mice.
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Summary
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Protein expression analysis:
Human CXCL7 and CXCL4 were detectable in serum of homozygous B-hCXCL7/hCXCL4 mice but not in wild-type mice, and mouse CXCL7 and CXCL4 were detectable only in wild-type mice.
